Volume 20, Issue 45, January - June, 2026

Purification of ginsenoside glycosidase-BglPm using ITC of Elastin like protein-tagged system

Hyok-Chol Ri¹, Jong-Hyok Kim¹, Chang-Bong Choe², Gyong- Jin Mun¹, Il-Tae Kim¹, Shu-Yang Dang³, Su-Chol Rim¹, Kwang-Il To^1♦

¹Institute of Chemistry and Biology, University of Science, Pyongyang, DPR Korea
²Faculty of Medical technology, Pyongsong Medical University, Pyongsong, DPR Korea
³College of Life Science, Northeast Forestry University, Harbin 150040, China

^♦Corresponding Author
Kwang-Il To, Institute of Chemistry and Biology, University of Sciences, Pyongyang, DPR Korea

ABSTRACT

Rare ginsenosides are known to have many medicinal values such as anti-tumor and anti-oxidant activity. Rare ginsenosides can be obtained by hydrolyzing saponin glycosides in ginseng with ginsenoside glycosidase. Glycosidases with high purity and activity are necessary to increase the efficiency of saponin conversion. A good example of effective purification is to utilize the elastin-like polypeptide (ELP) system. This purification method has the advantages of high purity, low cost and simple operation. In this study, we optimized the purification conditions of BglPm- ELP by the inverse transition cycle (ITC) method. In addition, we also investigated the enzymatic characteristics of the enzyme.

Keywords: Elastin-type polypeptides, Glycosidases, Ginsenoside, Protein purification

Drug Discovery, 2026, 20(45), e15dd3087

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DOI: https://doi.org/10.54905/disssi.v20i45.e15dd3087

Published: 03 June 2026